2NS0
Crystal structure of protein RHA04536 from Rhodococcus sp
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-21 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97907, 0.97921 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 37.683, 53.165, 90.062 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.040 - 2.005 |
R-factor | 0.19016 |
Rwork | 0.189 |
R-free | 0.21590 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.682 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.050 | 0.110 |
Number of reflections | 6142 | |
<I/σ(I)> | 33.36 | 11.5 |
Completeness [%] | 96.8 | 77 |
Redundancy | 6.5 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | PEG3350 25% , Bis-Tris 0.1M pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |