2NRX
Crystal structure of the C-terminal half of UvrC, in the presence of sulfate molecules
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-20 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.359, 81.015, 99.619 |
Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.900 |
R-factor | 0.187 |
Rwork | 0.184 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nrr |
RMSD bond length | 0.016 |
RMSD bond angle | 1.606 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.071 | 0.337 |
Number of reflections | 42886 | |
<I/σ(I)> | 12.2 | |
Completeness [%] | 97.2 | 94.8 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 1.8 M ammonium sulfate, 0.05 M Tris (pH 8.5), 0.025 M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |