2NRQ
Crystal structure of protein SSO0741 from Sulfolobus solfataricus, Pfam DUF54
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-15 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | H 3 2 |
Unit cell lengths | 127.540, 127.540, 61.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.430 - 2.600 |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.30300 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.055 | 0.354 |
Number of reflections | 6695 | |
<I/σ(I)> | 14.1 | 3.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.2 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | Sodium Formate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |