2NRN
Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-02-24 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 20.117, 87.875, 35.509 |
Unit cell angles | 90.00, 103.83, 90.00 |
Refinement procedure
Resolution | 43.940 - 1.400 |
R-factor | 0.18506 |
Rwork | 0.183 |
R-free | 0.21912 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ipz |
RMSD bond length | 0.009 |
RMSD bond angle | 1.148 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.940 | 1.430 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.057 | 0.451 |
Number of reflections | 22711 | |
<I/σ(I)> | 14.1 | 3 |
Completeness [%] | 95.8 | 91.5 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 295 | 0.1M sodium citrate, 1M ammonium dihydrophosphate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |