2NRL
Blackfin tuna myoglobin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-05 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.985, 51.976, 38.781 |
Unit cell angles | 90.00, 103.96, 90.00 |
Refinement procedure
Resolution | 30.000 - 0.910 |
R-factor | 0.1487 |
Rwork | 0.149 |
R-free | 0.14870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1myt |
RMSD bond length | 0.014 |
RMSD bond angle | 1.445 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 0.940 |
High resolution limit [Å] | 0.910 | 0.910 |
Number of reflections | 92131 | |
<I/σ(I)> | 16 | 3.1 |
Completeness [%] | 98.2 | 82.5 |
Redundancy | 7.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M potassium/sodium tartrate, 0.1 M sodium citrate, and 2.0 M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |