2NRK
Crystal structure of conserved protein GrpB from Enterococcus faecalis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-26 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97908, 0.97925 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.665, 82.149, 30.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.670 - 1.650 |
| R-factor | 0.17943 |
| Rwork | 0.178 |
| R-free | 0.21340 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.368 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.670 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 21900 | |
| <I/σ(I)> | 12.9 | 2.96 |
| Completeness [%] | 95.0 | 65 |
| Redundancy | 9.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2M MgCl2(6H2O), 0.1M HEPES pH 7.5, 22% Polyacrylic Acid 5100 Sodium salt, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
