2NRE
Crystal structure of pseudoudirinde synthase TruA in complex with leucyl tRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 80.355, 80.355, 205.373 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 4.000 |
R-factor | 0.26462 |
Rwork | 0.259 |
R-free | 0.35735 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1DJ0 and 1EHZ |
RMSD bond length | 0.012 |
RMSD bond angle | 1.327 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 4.140 |
High resolution limit [Å] | 4.000 | 4.000 |
Number of reflections | 6998 | |
<I/σ(I)> | 9.53 | 3.38 |
Completeness [%] | 99.2 | 97.9 |
Redundancy | 7.6 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG 3350, 0.2 M K3 Citrate, 5 mM spermine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | K3 Citrate | ||
3 | 1 | 1 | spermine | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 3350 | ||
6 | 1 | 2 | K3 Citrate | ||
7 | 1 | 2 | spermine | ||
8 | 1 | 2 | H2O |