2NRE
Crystal structure of pseudoudirinde synthase TruA in complex with leucyl tRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.355, 80.355, 205.373 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 4.000 |
| R-factor | 0.26462 |
| Rwork | 0.259 |
| R-free | 0.35735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DJ0 and 1EHZ |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.327 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 6998 | |
| <I/σ(I)> | 9.53 | 3.38 |
| Completeness [%] | 99.2 | 97.9 |
| Redundancy | 7.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG 3350, 0.2 M K3 Citrate, 5 mM spermine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | K3 Citrate | ||
| 3 | 1 | 1 | spermine | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | K3 Citrate | ||
| 7 | 1 | 2 | spermine | ||
| 8 | 1 | 2 | H2O |
