2NR7
Structural Genomics, the crystal structure of putative secretion activator protein from Porphyromonas gingivalis W83
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-13 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.537, 36.386, 62.474 |
| Unit cell angles | 90.00, 119.75, 90.00 |
Refinement procedure
| Resolution | 26.930 - 1.300 |
| R-factor | 0.16419 |
| Rwork | 0.162 |
| R-free | 0.19665 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.324 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.930 | 1.310 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.064 | 0.410 |
| Number of reflections | 42196 | |
| <I/σ(I)> | 38 | 2.92 |
| Completeness [%] | 92.5 | 68.4 |
| Redundancy | 6.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.1M Bis Tris pH5.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
