2NQW
Structure of the transporter associated domain from PG_0272, a CBS domain protein from Porphyromonas gingivalis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-29 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97910, 0.97929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.733, 45.195, 57.214 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.170 - 1.300 |
| R-factor | 0.13157 |
| Rwork | 0.130 |
| R-free | 0.16715 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.601 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.200 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 20972 | |
| <I/σ(I)> | 11.6 | 3.6 |
| Completeness [%] | 94.6 | 69 |
| Redundancy | 7.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 291 | 0.1M citric acid, 25% PEG 3350, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
