2NQH
High Resolution crystal structure of Escherichia coli endonuclease IV (Endo IV) E261Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-07-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.800, 59.200, 50.900 |
Unit cell angles | 90.00, 111.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.100 |
Rwork | 0.155 |
R-free | 0.19200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qtw |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.100 |
Number of reflections | 102718 |
<I/σ(I)> | 19.2 |
Completeness [%] | 92.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 298 | 20% MPEG-2000, 0.1M HEPES pH 5.9, 0.1mM ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |