2NQB
Drosophila Nucleosome Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.143, 109.581, 182.045 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 2.300 |
| Rwork | 0.223 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.956 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.045 | 0.302 |
| Number of reflections | 93949 | |
| <I/σ(I)> | 22 | 3 |
| Completeness [%] | 98.2 | 85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | Constituents of the crystallization buffer: Potassium Chloride, Manganese Chloride, and Potassium Cacodylate., pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Potassium Chloride | ||
| 2 | 1 | 1 | Manganese Chloride | ||
| 3 | 1 | 1 | Potassium Cacodylate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Potassium Chloride | ||
| 6 | 1 | 2 | Manganese Chloride | ||
| 7 | 1 | 2 | Potassium Cacodylate | ||
| 8 | 1 | 2 | H2O |
