2NQ5
Crystal structure of methyltransferase from Streptococcus mutans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-27 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.291, 99.194, 78.619 |
| Unit cell angles | 90.00, 109.25, 90.00 |
Refinement procedure
| Resolution | 24.960 - 1.900 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1h |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.056 |
| Number of reflections | 58104 |
| Completeness [%] | 97.5 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 25% PEG 3350, 0.1M Bis Tris, 0.2M magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
