2NQ2

An inward-facing conformation of a putative metal-chelate type ABC transporter.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-02-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97925, 0.89194, 0.97927
Spacegroup name	P 21 21 21
Unit cell lengths	97.846, 142.465, 150.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 2.400
Rwork	0.222
R-free	0.25970
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.185
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	82020
<I/σ(I)>	16.1
Completeness [%]	99.5	99.2
Redundancy	14.5	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	34% pentaerythritol propoxylate, 0.1M sodium citrate, pH 5.5, 0.2M potassium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	34% pentaerythritol propoxylate, 0.1M sodium citrate, pH 5.5, 0.2M potassium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K