2NQ2
An inward-facing conformation of a putative metal-chelate type ABC transporter.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-02-16 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.97925, 0.89194, 0.97927 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.846, 142.465, 150.268 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.000 - 2.400 |
Rwork | 0.222 |
R-free | 0.25970 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.185 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 82020 | |
<I/σ(I)> | 16.1 | |
Completeness [%] | 99.5 | 99.2 |
Redundancy | 14.5 | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 34% pentaerythritol propoxylate, 0.1M sodium citrate, pH 5.5, 0.2M potassium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 34% pentaerythritol propoxylate, 0.1M sodium citrate, pH 5.5, 0.2M potassium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K |