2NPZ
Crystal structure of junctioned hairpin ribozyme incorporating synthetic propyl linker
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.130, 91.130, 144.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.660 - 3.350 |
| R-factor | 0.268 |
| Rwork | 0.261 |
| R-free | 0.27700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2.05 angstrom junctionless ribozyme |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.660 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 5453 | |
| <I/σ(I)> | 16.2 | 4.7 |
| Completeness [%] | 98.6 | 99.1 |
| Redundancy | 11.3 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 2000 MME, lithium sulfate, cacodylate, spermidine, cobalt hexaamine, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6.0 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2000 MME | ||
| 2 | 1 | 1 | lithium sulfate | ||
| 3 | 1 | 1 | cacodylate | ||
| 4 | 1 | 1 | spermidine | ||
| 5 | 1 | 1 | cobalt hexaamine | ||
| 6 | 1 | 2 | PEG 2000 MME | ||
| 7 | 1 | 2 | cobalt hexaamine |
