2NPM
crystal structure of Cryptosporidium parvum 14-3-3 protein in complex with peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-22 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 104.125, 104.125, 148.877 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.680 - 2.520 |
| R-factor | 0.22174 |
| Rwork | 0.221 |
| R-free | 0.27541 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ywt |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.063 | 0.981 |
| Number of reflections | 28423 | |
| <I/σ(I)> | 10.4 | 2.36 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.3 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 14% PEG 3350, 0.1 M Calcium Acetate, 0.2 M Trimethylamine-N-oxide, 0.1 M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
