2NP9
Crystal structure of a dioxygenase in the Crotonase superfamily
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-13 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 139.860, 156.660, 171.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.450 |
| R-factor | 0.328 |
| Rwork | 0.328 |
| R-free | 0.35600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNBOUND FORM OF THE ENZYME |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.483 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.577 |
| Number of reflections | 143933 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 98.0 | 96.7 |
| Redundancy | 1.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 293 | pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 5.60 |
