2NOK
Crystal Structure of an RNA domain from Hepatitis C virus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.098, 65.351, 92.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.25932 |
| Rwork | 0.257 |
| R-free | 0.30167 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.833 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 7158 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20mM MgSO4, 100mM KCl, 30mM MnCl2, 2-6% MPD over 40mM MgSO4, 200mM KCl and 40-50% MPD., pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgSO4 | ||
| 10 | 1 | 2 | MgSO4 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | MnCl2 | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | MgSO4 | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | MgSO4 | ||
| 8 | 1 | 2 | KCl | ||
| 9 | 1 | 2 | MnCl2 |
