2NND
The Structural Identification of the Interaction Site and Functional State of RBP for its Membrane Receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.488 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 53.636, 53.636, 137.347 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.300 - 1.600 |
R-factor | 0.198 |
Rwork | 0.190 |
R-free | 0.22741 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qy0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.224 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.300 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.093 | 0.306 |
Number of reflections | 27306 | |
<I/σ(I)> | 3.8 | 2.4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.5 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 291 | CdCl, malate buffer, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |