2NN4
Crystal structure of Bacillus subtilis yqgQ, Pfam DUF910
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-14 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.850, 41.250, 55.180 |
Unit cell angles | 90.00, 113.39, 90.00 |
Refinement procedure
Resolution | 47.590 - 2.100 |
R-factor | 0.247 |
Rwork | 0.247 |
R-free | 0.28000 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.080 | 0.370 |
Number of reflections | 12344 | |
<I/σ(I)> | 12.2 | 2.8 |
Completeness [%] | 97.5 | 84.7 |
Redundancy | 6.5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 278 | 0.066M sodium di-hydrogen phophate, 3.385M di-potassium hydrogen phosphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 278K |