2NN2
Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.978 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.143, 72.143, 116.048 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.310 - 2.100 |
R-factor | 0.21647 |
Rwork | 0.214 |
R-free | 0.26749 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1buo |
RMSD bond length | 0.017 |
RMSD bond angle | 1.642 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.310 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 19243 | |
<I/σ(I)> | 15.45 | 3.08 |
Completeness [%] | 96.7 | 95.6 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 0.7 M sodium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |