2NMS

The Crystal Structure of the Extracellular Domain of the Inhibitor Receptor Expressed on Myeloid Cells IREM-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-09
Detector	MAR CCD 225 mm
Wavelength(s)	0.873
Spacegroup name	P 31 2 1
Unit cell lengths	54.230, 54.230, 72.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.670 - 2.600
Rwork	0.218
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zox
RMSD bond length	0.008
RMSD bond angle	1.700
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.670	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.097	0.325
Number of reflections	3936
<I/σ(I)>	0.0967	4.17
Completeness [%]	97.8	95.7
Redundancy	4.93	5.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	0.25M potassium thiocyanate, 25% (w/v) PEG MME 2000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K