2NLX
Crystal structure of the apo E. coli xylulose kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-05-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.780, 112.020, 138.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.880 - 2.700 |
| R-factor | 0.20681 |
| Rwork | 0.205 |
| R-free | 0.25417 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.706 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.880 | 24.880 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 29677 | |
| Completeness [%] | 10.1 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | Drops containing equal volume of 26 mg/ml protein solution mixed with the precipitant solution containing 1.5 M ammonium sulfate, 50 mM sodium citrate, 1% w/v t-butanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
