2NLV
CRYSTAL STRUCTURE OF A XISI-LIKE PROTEIN (AVA_3825) FROM ANABAENA VARIABILIS ATCC 29413 AT 1.30 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000, 0.9795, 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.779, 37.354, 63.453 |
| Unit cell angles | 90.00, 97.22, 90.00 |
Refinement procedure
| Resolution | 29.099 - 1.300 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.19500 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.758 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.099 | 29.100 | 1.330 |
| High resolution limit [Å] | 1.300 | 5.810 | 1.300 |
| Rmerge | 0.062 | 0.029 | 0.618 |
| Total number of observations | 2252 | 13820 | |
| Number of reflections | 52812 | ||
| <I/σ(I)> | 7.9 | 15.1 | 1.2 |
| Completeness [%] | 98.2 | 98.6 | 96.3 |
| Redundancy | 3.6 | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | 1.0M LiCl, 20.0% PEG-6000, 0.1M TRIS, pH 8.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
