2NLG
Human beta-defensin-1 (Mutant Lys22Glu)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.780, 33.180, 41.940 |
| Unit cell angles | 73.60, 85.30, 86.50 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ijv |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.601 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.041 | 0.156 |
| Number of reflections | 14844 | |
| <I/σ(I)> | 16 | 5.3 |
| Completeness [%] | 92.6 | 87.1 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | PEG 8000, AMMONIUM SULFATE, SODIUM CACODYLATE, pH 6.5, vapor diffusion, temperature 293K |
