2NLC
Human beta-defensin-1 (mutant Ser8Ala)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.740, 33.190, 41.850 |
| Unit cell angles | 73.90, 85.50, 86.20 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ijv |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.666 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.051 | 0.207 |
| Number of reflections | 14783 | |
| <I/σ(I)> | 29 | 6.4 |
| Completeness [%] | 92.6 | 85.8 |
| Redundancy | 6.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | PEG 8000, LITHIUM SULFATE, pH 7.0, vapor diffusion, temperature 293K |
