2NG1

N AND GTPASE DOMAINS OF THE SIGNAL SEQUENCE RECOGNITION PROTEIN FFH FROM THERMUS AQUATICUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-12-12
Detector	RIGAKU RAXIS
Spacegroup name	C 1 2 1
Unit cell lengths	110.425, 54.214, 58.465
Unit cell angles	90.00, 119.62, 90.00

Refinement procedure

Resolution	20.000 - 2.020
R-factor	0.2
Rwork	0.200
R-free	0.29100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ffh
RMSD bond length	0.010
RMSD bond angle	24.150 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.090
High resolution limit [Å]	2.020	2.020
Rmerge	0.072	0.284
Number of reflections	18481
<I/σ(I)>	12.9	3.6
Completeness [%]	93.0	92.8 *
Redundancy	3.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	GDP	2 (mM)
3	1	reservoir	dioxane	35 (%(v/v))