2NCD
NCD (NON-CLARET DISJUNCTIONAL) DIMER FROM D. MELANOGASTER
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-15 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 123.000, 123.000, 121.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NCD MONOMER |
RMSD bond length | 0.007 |
RMSD bond angle | 12.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.500 |
High resolution limit [Å] | 2.500 | 2.300 |
Rmerge | 0.068 | 0.130 |
Total number of observations | 190358 * | |
Number of reflections | 18795 | |
<I/σ(I)> | 15 | 5 |
Completeness [%] | 98.6 | 92.6 |
Redundancy | 10.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | THE MOTHER LIQUOR CONTAINED PROTEIN AT ABOUT 20 MG/ML, 2 MM ADP, 10 MM MGCL2, 100 MM NACL, 700 MM LI2SO4, 1 MM EGTA, AND 1 MM DTT IN 20 MM HEPES, PH 7.5. THE RESERVOIR WAS 1.4 M LI2SO4, 10 MM MGCL2, 1 MM EGTA, 1 MM DTT IN 20 MM HEPES, PH 7.5., VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
10 | 1 | reservoir | 10 (mM) | ||
11 | 1 | reservoir | EGTA | 1 (mM) | |
12 | 1 | reservoir | dithiothreitol | 1 (mM) | |
13 | 1 | reservoir | HEPES | 20 (mM) | |
2 | 1 | drop | ADP | 2 (mM) | |
3 | 1 | drop | 10 (mM) | ||
4 | 1 | drop | 100 (mM) | ||
5 | 1 | drop | 700 (mM) | ||
6 | 1 | drop | EGTA | 1 (mM) | |
7 | 1 | drop | dithiothreitol | 1 (mM) | |
8 | 1 | drop | HEPES | 20 (mM) | |
9 | 1 | reservoir | 1.4 (M) |