2NA4

Curli secretion specificity factor CsgE W48A/F79A mutant

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE	Bruker Avance	600
2	Bruker	DMX	Bruker DMX	750
3	Bruker	AVANCE	Bruker Avance	800

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	2	3D C(CO)NH
11	1	2	3D H(CCO)NH
12	1	1	3D 1H-15N NOESY
13	1	2	3D 1H-13C NOESY
14	1	2	3D 1H-13C NOESY aromatic
2	1	2	2D 1H-13C HSQC
3	1	2	2D 1H-13C HSQC aromatic
4	1	2	2D 1H-13C HSQC aliphatic
5	1	2	3D CBCA(CO)NH
6	1	2	3D C(CO)NH
7	1	2	3D HNCO
8	1	2	3D HNCACB
9	1	2	3D HN(CO)CA

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	5.8	ambient	298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	20