2N3O
Structure of PTB RRM1(41-163) bound to an RNA stemloop containing a structured loop derived from viral internal ribosomal entry site RNA
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | Bruker Avance | 500 |
| 2 | Bruker | AVANCE | Bruker Avance | 600 |
| 3 | Bruker | AVANCE | Bruker Avance | 700 |
| 4 | Bruker | AVANCE | Bruker Avance | 750 |
| 5 | Bruker | AVANCE | Bruker Avance | 900 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 2 | 3D HNCA |
| 10 | 1 | 5 | 2D 1H-1H TOCSY |
| 11 | 1 | 5 | 2D 1H-1H NOESY |
| 12 | 1 | 3 | 3D f1-13Cfiltered,f2-13C-edited NOESY aliphatic-HSQC |
| 13 | 1 | 4 | 3D f1-13Cfiltered,f2-13C-edited NOESY aliphatic-HSQC |
| 14 | 1 | 3 | 2D f2-13C-filtered NOESY |
| 15 | 3 | 2 | 3D aliphatic-HSQC-NOESY |
| 16 | 1 | 3 | 2D H2C2 TROSY |
| 17 | 1 | 3 | 2D H6C6 TROSY |
| 18 | 1 | 3 | 2D H8C8 TROSY |
| 19 | 1 | 8 | 2D H2C2 TROSY |
| 2 | 1 | 2 | 3D HN(CO)CA |
| 20 | 1 | 8 | 2D H6C6 TROSY |
| 21 | 1 | 8 | 2D H8C8 TROSY |
| 22 | 1 | 3 | 2D H5C5 TROSY |
| 23 | 1 | 3 | 2D H1'C1' TROSY |
| 24 | 1 | 8 | 2D H5C5 TROSY |
| 25 | 1 | 8 | 2D H1'C1' TROSY |
| 26 | 1 | 1 | 2D HN TROSY |
| 27 | 1 | 7 | 2D HN TROSY |
| 28 | 3 | 9 | 2D HN TROSY |
| 29 | 3 | 9 | 2D HN TROSY |
| 3 | 1 | 2 | 3D HNCACB |
| 30 | 1 | 1 | 2D {1H}15N-NOE HSQC |
| 31 | 3 | 1 | 2D 1H-1H NOESY |
| 32 | 1 | 12 | 3D f1-13Cfiltered,f2-13C-edited NOESY aliphatic-HSQC |
| 33 | 1 | 12 | 3D 1H-13C NOESY aliphatic |
| 34 | 1 | 4 | 3D 1H-13C NOESY aliphatic |
| 35 | 1 | 11 | 3D f1-13Cfiltered,f2-13C-edited NOESY aliphatic-HSQC |
| 36 | 1 | 4 | 2D 1H-13C HSQC aliphatic |
| 37 | 1 | 3 | 2D 1H-13C HSQC aromatic |
| 38 | 1 | 3 | 3D 1H-13C NOESY aliphatic |
| 39 | 1 | 5 | 3D 1H-13C NOESY aromatic |
| 4 | 1 | 2 | 3D HNCO |
| 40 | 4 | 13 | 3D 1H-13C NOESY aliphatic |
| 41 | 4 | 13 | 3D 1H-13C NOESY aromatic |
| 42 | 1 | 11 | 2D 1H-13C HSQC aliphatic |
| 43 | 3 | 14 | 2D 1H-1H NOESY |
| 5 | 1 | 2 | 3D HCCH-TOCSY |
| 6 | 1 | 2 | 3D HCCH-TOCSY |
| 7 | 1 | 1 | 3D 1H-15N NOESY-HSQC |
| 8 | 1 | 2 | 3D 1H-13C NOESY aliphatic-HSQC |
| 9 | 1 | 2 | 3D 1H-13C NOESY aromatic-HSQC |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.5 | ambient | 313 |
| 2 | 6.5 | ambient | 298 |
| 3 | 6.5 | ambient | 278 |
| 4 | 6.5 | ambient | 303 |
Conformers
| Conformers Calculated Total Number | 250 |
| Conformers Submitted Total Number | 20 |
