2N32

NMR solution structure of the N-terminal domain of NisI, a lipoprotein from Lactococcus lactis which confers immunity against nisin

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE	Bruker Avance	600
2	Bruker	AVANCE	Bruker Avance	700
3	Bruker	AVANCE	Bruker Avance	800
4	Bruker	AVANCE	Bruker Avance	950
5	Bruker	AVANCE	Bruker Avance	900

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	1	3D 1H-15N NOESY
11	1	2	3D 1H-13C NOESY aliphatic
12	1	2	3D 1H-13C NOESY aromatic
13	1	2	3D HNCACO
2	1	2	2D 1H-13C HSQC aliphatic
3	1	2	2D 1H-13C HSQC aromatic
4	1	2	3D CBCA(CO)NH
5	1	2	3D HNCO
6	1	2	3D HNCACB
7	1	2	3D HBHA(CO)NH
8	1	2	3D H(CCO)NH
9	1	2	3D C(CO)NH

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.5	1	298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	20