2N03
Solution NMR Structure plectin repeat domain 6 (4403-4606) of Plectin from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR6354E
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Varian | INOVA | Varian INOVA | 750 |
| 2 | Varian | DDR2 | Varian DDR2 | 600 |
| 3 | Bruker | AMX | Bruker AMX | 900 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 1H-15N HSQC |
| 10 | 1 | 1 | 3D [H]-NOESY-[NH/CH] ( mix= 250 ms) |
| 11 | 1 | 1 | 3D [(H)C]-NOESY-[CH], ( mix = 250 ms) |
| 12 | 1 | 2 | 3D (H)CCH-COSY, aliphatic |
| 13 | 1 | 2 | 3D (H)CCH-TOCSY, aliphatic |
| 14 | 1 | 2 | 3D [H]-NOESY-[NH/CH] ( mix = 70 ms) # |
| 15 | 1 | 3 | 2D ct [13C,1H] HSQC (ct delay 28 ms) |
| 16 | 1 | 3 | 2D ct [13C,1H] HSQC (ct delay 42 ms) |
| 17 | 1 | 3 | 2D ct [13C,1H] HSQC (ct delay 56 ms) |
| 2 | 1 | 2 | 2D 1H-13C HSQC aliphatic |
| 3 | 1 | 2 | 2D 1H-13C HSQC aromatic |
| 4 | 1 | 1 | 3D TROSY-HNCO |
| 5 | 1 | 1 | 3D TROSY-HNCA |
| 6 | 1 | 1 | 3D TROSY-HN(CO)CA |
| 7 | 1 | 1 | 3D TROSY-HNCACB |
| 8 | 1 | 1 | 3D HMCM[CG]CBCA |
| 9 | 1 | 1 | 3D HMCMCBCA |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.5 | ambient | 298 |
Conformers
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
