2MYS

MYOSIN SUBFRAGMENT-1, ALPHA CARBON COORDINATES ONLY FOR THE TWO LIGHT CHAINS

Replaces:  1MYS

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1991
Detector	SIEMENS
Spacegroup name	C 2 2 21
Unit cell lengths	98.400, 124.200, 274.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
Rwork	0.223
RMSD bond length	0.018 *
RMSD bond angle	2.500 *
Data reduction software	XDS
Data scaling software	CCP4
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	4.500 *
High resolution limit [Å]	2.800 *
Rmerge	0.092 *
Total number of observations	178986 *
Number of reflections	36781 *
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6.7 *		macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium salfate	1.35 (M)
2	1	1	potassium chloride	500 (mM)
3	1	1	potassium phosphate	50 (mM)
4	1	2	dithiothreitol	5 (mM)
5	1	2		0.5 (mM)
6	1	2	prtein	8-12 (mg/ml)