2MUC
MUCONATE CYCLOISOMERASE VARIANT F329I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | I 4 |
| Unit cell lengths | 139.100, 139.100, 84.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.21600 |
| Structure solution method | OTHER |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.097 |
| Number of reflections | 120580 |
| Completeness [%] | 79.1 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | double dialysis * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | MES | 20 (mM) | |
| 2 | 1 | 1 | 2 (mM) | ||
| 3 | 1 | 1 | 50 (mM) | ||
| 4 | 1 | 1 | 0.02 (%(w/v)) | ||
| 5 | 1 | 1 | beta-mercaptoethanol | 7 (mM) | |
| 6 | 1 | 1 | protein | 8 (mg/ml) |
