2MTJ

NMR structure of the III-IV-V three-way junction from the VS ribozyme

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Varian	UNITY	Varian Unity	600

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	1D flip-back watergate 1H
10	2	3	2D H(NC)-TOCSY-(C)H for guanosine residues
11	2	2	2D 1H-13C HMQC
12	2	2	2D 1H-15N MQ-(HC)N(C)H
13	1	1	2D 1H-15N CPMG-NOESY
14	1	1	2D HNN-COSY
2	1	1	2D 1H-15N HSQC
3	1	1	2D 1H-15N HSQC NH2 only
4	1	1	3D 1H-15N NOESY
5	2	2	2D 1H-13C HSQC
6	2	2	3D 13C-edited HMQC-NOESY
7	2	2	3D CT-HCCH-COSY
8	2	2	3D HCCH-TOCSY
9	2	3	3D 15N-edited NOESY-HSQC

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.5		288
2	6.5		298

Conformers

Conformers Calculated Total Number	200
Conformers Submitted Total Number	21