2MPR
MALTOPORIN FROM SALMONELLA TYPHIMURIUM
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE M18X |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-09-04 |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 124.500, 211.800, 184.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mpr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.400 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.350 * |
| Total number of observations | 278202 * | |
| Number of reflections | 90607 | |
| <I/σ(I)> | 12 | 4.3 |
| Completeness [%] | 88.0 * | 62 * |
| Redundancy | 3.1 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 18 * | MALTOPORIN WAS CRYSTALLIZED BY HANGING-DROP METHOD. DROP: 5-8 MG/ML PROTEIN, 0.3% C8E4, 0.8% C6DAO, 1 MM CACL2, 1MM MGCL2, 14-18% PEG 1500, 0.02% NAN3 RESERVOIR: 28-32% PEG 1500, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-8 (mg/ml) | |
| 2 | 1 | drop | n-octyltetraoxyethylene | 0.3 (%) | |
| 3 | 1 | drop | n-hexyldimethylaminoxide | 0.8 (%) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | drop | PEG1500 | 14-18 (%) | |
| 7 | 1 | drop | 0.02 (%) | ||
| 8 | 1 | reservoir | PEG1500 | 28-32 (%) |
