2MPA

BACTERICIDAL ANTIBODY AGAINST NEISSERIA MENINGITIDIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-03-15
Detector	MAC Science DIP-2020
Spacegroup name	P 1 21 1
Unit cell lengths	55.680, 69.100, 72.950
Unit cell angles	90.00, 112.01, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.202
Rwork	0.202
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2HFL
RMSD bond length	0.008
RMSD bond angle	1.533
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.054	0.357
Number of reflections	15404
<I/σ(I)>	16.8	2.3
Completeness [%]	97.0	96.5
Redundancy	6.1	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6		THE MN12H2 FAB PEPTIDE COMPLEX WAS CRYSTALLIZED FROM 15% W/V PEG 20000, 100 MM SODIUM ACETATE, PH 4.6, 20 MM CDCL2, VAPOR DIFFUSION, HANGING DROP