2MPA
BACTERICIDAL ANTIBODY AGAINST NEISSERIA MENINGITIDIS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1995-03-15 |
Detector | MAC Science DIP-2020 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.680, 69.100, 72.950 |
Unit cell angles | 90.00, 112.01, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2HFL |
RMSD bond length | 0.008 |
RMSD bond angle | 1.533 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.054 | 0.357 |
Number of reflections | 15404 | |
<I/σ(I)> | 16.8 | 2.3 |
Completeness [%] | 97.0 | 96.5 |
Redundancy | 6.1 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.6 | THE MN12H2 FAB PEPTIDE COMPLEX WAS CRYSTALLIZED FROM 15% W/V PEG 20000, 100 MM SODIUM ACETATE, PH 4.6, 20 MM CDCL2, VAPOR DIFFUSION, HANGING DROP |