2MME
Hybrid structure of the Shigella flexneri MxiH Type three secretion system needle
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLID-STATE NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | Bruker Avance | 850 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 13C-13C (PDSD) |
| 2 | 1 | 1 | 15N-13C (Specific-CP) |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 5.5 | ambient | 278 |
Conformers
| Conformers Calculated Total Number | 5000 |
| Conformers Submitted Total Number | 10 |
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | TVIPS TEMCAM-F415 (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 7.7 |
