2MLL
MISTLETOE LECTIN I FROM VISCUM ALBUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 107.648, 107.648, 311.278 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.248 * |
| Rwork | 0.251 |
| R-free | 0.31900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aai |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.066 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 10.000 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.048 * | 0.181 * |
| Total number of observations | 334925 * | |
| Number of reflections | 29232 | |
| Completeness [%] | 99.1 | 97.9 * |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | pH 4.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 40-45 (%) | |
| 3 | 1 | reservoir | 0.18 (M) | ||
| 4 | 1 | reservoir | acetate | 0.1 (M) | or 0.1M phosphate |
