2MKK
Structural model of tandem RRM domains of cytoplasmic polyadenylation element binding protein 1 (CPEB1) in complex with RNA
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | Avance | Bruker Avance | 900 |
| 2 | Bruker | Avance | Bruker Avance | 700 |
| 3 | Bruker | Avance | Bruker Avance | 600 |
| 4 | Bruker | Avance | Bruker Avance | 500 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 4 | 2D 1H-15N HSQC |
| 10 | 1 | 1 | 3D trCBCA(CO)NH |
| 11 | 1 | 1 | 3D trHNCACB |
| 12 | 1 | 1 | 3D trHNCACO |
| 13 | 1 | 1 | 3D trHNCO |
| 14 | 1 | 2 | 3D HCCH-TOCSY |
| 15 | 1 | 2 | 3D HCCH-TOCSY |
| 16 | 1 | 4 | 3D 1H-15N NOESY |
| 17 | 1 | 1 | 3D 1H-13C NOESY aliphatic |
| 18 | 1 | 1 | 3D 1H-13C NOESY aromatic |
| 19 | 1 | 6 | 2D 13C-F1-filtered F2-filtered NOESY |
| 2 | 1 | 4 | 2D TROSY |
| 20 | 1 | 6 | 2D 1H-1H F1-13C-filtered F2-13C-edited NOESY |
| 21 | 1 | 6 | 3D 13C-F1-edited F3-filtered NOESY HSQC |
| 3 | 1 | 2 | 2D 1H-13C HSQC |
| 4 | 1 | 2 | 2D 1H-13C HSQC aliphatic |
| 5 | 1 | 2 | 2D 1H-13C HSQC aromatic |
| 6 | 1 | 5 | 2D 1H-1H TOCSY |
| 7 | 1 | 5 | 2D 1H-1H NOESY |
| 8 | 1 | 1 | 3D trHNCA |
| 9 | 1 | 1 | 3D trHN(CO)CA |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.5 | ambient | 313 |
Conformers
| Conformers Calculated Total Number | 20 |
| Conformers Submitted Total Number | 10 |
