2MI0
NMR structure of the I-V kissing-loop interaction of the Neurospora VS ribozyme
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Varian | INOVA | Varian INOVA | 500 |
| 2 | Varian | INOVA | Varian INOVA | 600 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 1H-15N HSQC |
| 10 | 2 | 3 | 2D 1H-15N CPMG NOESY |
| 11 | 1 | 3 | 2D 1H-1H flip-back WATERGATE NOESY |
| 12 | 1 | 1 | 3D 15N-edited NOESY-HSQC |
| 13 | 1 | 3 | 3D 15N-edited NOESY-HSQC |
| 14 | 1 | 2 | 2D 1H-13C CT-HSQC |
| 15 | 1 | 4 | 2D 1H-13C CT-HSQC |
| 16 | 1 | 5 | 2D 1H-13C CT-HSQC |
| 17 | 1 | 6 | 2D 1H-13C CT-HSQC |
| 18 | 1 | 9 | 3D 15N/13C-edited NOESY-HSQC |
| 19 | 1 | 10 | 3D 15N/13C-edited NOESY-HSQC |
| 2 | 1 | 3 | 2D 1H-15N HSQC |
| 20 | 1 | 2 | 3D 13C-edited HMQC-NOESY |
| 21 | 1 | 4 | 3D 13C-edited HMQC-NOESY |
| 22 | 1 | 5 | 3D 13C-edited HMQC-NOESY |
| 23 | 1 | 6 | 3D 13C-edited HMQC-NOESY |
| 24 | 1 | 2 | 3D HCCH-COSY |
| 25 | 1 | 4 | 3D HCCH-COSY |
| 26 | 1 | 6 | 3D HCCH-COSY |
| 27 | 1 | 1 | 1D 15N-decoupled 1H WATERGATE |
| 28 | 1 | 3 | 1D 15N-decoupled 1H WATERGATE |
| 29 | 1 | 1 | 1D 15N-filtered 1H WATERGATE |
| 3 | 1 | 1 | 2D 1H-15N HSQC NH2 only |
| 30 | 1 | 3 | 1D 15N-filtered 1H WATERGATE |
| 31 | 1 | 1 | 1D 15N-edited 1H WATERGATE |
| 32 | 1 | 3 | 1D 15N-edited 1H WATERGATE |
| 33 | 1 | 11 | 2D 1H-15N HSQC |
| 4 | 1 | 3 | 2D 1H-15N HSQC NH2 only |
| 5 | 1 | 1 | 2D 1H-15N NHH COSY |
| 6 | 1 | 3 | 2D 1H-15N NHH COSY |
| 7 | 1 | 7 | 2D 1H-15N HMQC J=21Hz |
| 8 | 1 | 8 | 2D 1H-15N HMQC J=21Hz |
| 9 | 2 | 1 | 2D 1H-15N CPMG NOESY |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 7 | ambient | 298 |
| 2 | 7 | ambient | 288 |
Conformers
| Conformers Calculated Total Number | 500 |
| Conformers Submitted Total Number | 21 |
