2MC0

Structural Basis of a Thiopeptide Antibiotic Multidrug Resistance System from Streptomyces lividans:Nosiheptide in Complex with TipAS

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	DRX	Bruker DRX	600
2	Bruker	DRX	Bruker DRX	800

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	1	3D 1H-15N NOESY
11	3	3	3D 1H-13C NOESY aliphatic
12	3	3	3D 1H-13C NOESY aromatic
13	3	3	2D 1H-1H NOESY/filter against 13C-15N
14	2	2	2D 1H-1H NOESY/filter against 13C-15N
15	3	3	2D 1H-1H TOCSY/filter against 13C-15N
16	2	2	Doublet-separated 2D 1H-15N HSQC
17	4	4	Doublet-separated 2D 1H-15N HSQC
18	2	2	J-resolved ct 13C-HSQC
19	4	4	J-resolved ct 13C-HSQC
2	3	3	2D 1H-13C HSQC aliphatic
20	2	2	3D CBCANH
3	2	2	3D HNCO
4	2	2	3D CBCA(CO)NH
5	2	2	3D C(CO)NH
6	2	2	3D HBHA(CO)NH
7	2	2	3D HC(CO)NH TOCSY
8	3	3	3D HCCH-TOCSY
9	1	1	3D HNHA

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	5.9	ambient	298
2	5.9	ambient	298
3		ambient	298
4	5.9	ambient	298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	10