2M5E

Structure of the C-domain of Calcium-saturated Calmodulin bound to the IQ motif of NaV1.2

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE II	Bruker Avance II	500
2	Bruker	AVANCE II	Bruker Avance II	800

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	1	3D H(CCO)NH
11	1	1	3D 1H-15N NOESY
12	1	2	3D 1H-13C NOESY
13	1	2	3D HCCH-TOCSY
14	1	1	2D 1H-15N HSQC
15	1	2	2D 1H-13C HMQC
16	1	2	3D 1H-13C edited, 12C filtered NOESY
17	1	1	2D 1H-1H doubly 12C,14N filtered NOESY
18	1	1	2D 1H-1H 12C,14N filtered TOCSY
19	1	2	2D 1H-1H 12C filtered NOESY
2	1	2	2D 1H-13C HMQC
20	1	2	2D 1H-1H 12C,14N filtered TOCSY
3	1	1	3D HNCA
4	1	1	3D HN(CO)CA
5	1	1	3D HNCACB
6	1	1	3D HN(COCA)CB
7	1	1	3D HNCO
8	1	1	3D HN(CA)CO
9	1	1	3D C(CO)NH

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.8	ambient	298

Conformers

Conformers Calculated Total Number	300
Conformers Submitted Total Number	21