2M5A

Protein A binding by an engineered Affibody molecule

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Varian	INOVA	Varian INOVA	900
2	Varian	INOVA	Varian INOVA	800

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	3D HNCO
10	1	1	2D 1H-13C HSQC aliphatic
11	1	1	2D (HB)CB(CGCDCE)HE
12	1	1	3D 1H-15N NOESY
13	1	1	3D 1H-13C NOESY aliphatic
14	1	1	3D 1H-13C NOESY aromatic
15	1	1	2D F1(13C,15N)-filtered, F2(13C)-edited NOESY
16	1	2	3D HNCO
17	1	2	3D HNCA
18	1	2	3D HN(CO)CA
19	1	2	3D HNCACB
2	1	1	3D HNCA
20	1	2	3D HN(COCA)CB
21	1	2	3D 1H-15N TOCSY
22	1	2	3D HCCH-COSY
23	1	2	3D HCCH-TOCSY
24	1	2	2D 1H-15N HSQC
25	1	2	2D 1H-13C HSQC aliphatic
26	1	2	2D (HB)CB(CGCDCE)HE
27	1	2	3D 1H-15N NOESY
28	1	2	3D 1H-13C NOESY aliphatic
29	1	2	3D 1H-13C NOESY aromatic
3	1	1	3D HN(CO)CA
30	1	2	2D F1(13C,15N)-filtered, F2(13C)-edited NOESY
4	1	1	3D HNCACB
5	1	1	3D HN(COCA)CB
6	1	1	3D 1H-15N TOCSY
7	1	1	3D HCCH-COSY
8	1	1	3D HCCH-TOCSY
9	1	1	2D 1H-15N HSQC

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	5.6	ambient	298

Conformers

Conformers Calculated Total Number	50
Conformers Submitted Total Number	20