2M3T

Solution-state NMR structure of wild-type human gamma(S)-crystallin

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Varian	INOVA	Varian INOVA	800

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	2	2D 1H-15N HSQC
10	1	2	3D 1H-13C NOESY aliphatic
11	1	2	3D 1H-13C NOESY aromatic
12	2	1	2D 1H-15N HSQC
2	1	2	2D 1H-13C HSQC aliphatic
3	1	2	2D 1H-13C HSQC aromatic
4	1	2	3D HNCACB
5	1	2	3D CBCA(CO)NH
6	1	2	3D HNCO
7	1	2	3D HCCH-TOCSY
8	1	2	3D HCCH-COSY
9	1	2	3D 1H-15N NOESY

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	4.5	ambient	295
2	4.5	ambient	305

Conformers

Conformers Calculated Total Number	200
Conformers Submitted Total Number	21