2M1V
NMR solution structure of the d3'-hairpin from the Sc.ai5gamma group II intron including the EBS1:dIBS1 RNA:DNA hybrid
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | Bruker Avance | 700 |
| 2 | Bruker | AVANCE | Bruker Avance | 600 |
| 3 | Bruker | AVANCE | Bruker Avance | 500 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 1H-1H NOESY |
| 10 | 3 | 2 | 2D 1H-1H NOESY |
| 11 | 2 | 2 | 2D 1H-1H NOESY |
| 12 | 1 | 6 | 2D 1H-13C HSQC aliphatic |
| 13 | 1 | 6 | 2D 1H-13C HSQC aromatic |
| 14 | 1 | 1 | 1D 31P |
| 15 | 1 | 3 | F1, F2 X-filtered 1H-1H NOESY |
| 16 | 1 | 3 | F1, F2 X-filtered 1H-1H TOCSY |
| 2 | 1 | 1 | 2D 1H-1H TOCSY |
| 3 | 1 | 5 | 2D 1H-1H NOESY |
| 4 | 1 | 4 | 2D 1H-1H NOESY |
| 5 | 1 | 4 | 2D 1H-1H TOCSY |
| 6 | 1 | 3 | 2D 1H-15N HSQC |
| 7 | 2 | 2 | 2D 1H-15N HSQC |
| 8 | 1 | 6 | 2D 1H-13C HSQC aliphatic |
| 9 | 1 | 6 | 2D 1H-13C HSQC aromatic |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.8 | ambient | 298 |
| 2 | 6.8 | ambient | 278 |
| 3 | 6.8 | ambient | 293 |
Conformers
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 18 |
