2LKF

LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-02
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	49.220, 49.520, 127.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.500
R-factor	0.227
Rwork	0.227
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PREVIOUSLY DETERMINED STRUCTURE
RMSD bond length	0.011
RMSD bond angle	27.561 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.048 *	0.123 *
Number of reflections	12697
Completeness [%]	88.9	54.1
Redundancy	5.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	20 *	pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3000	25-30 (%(w/v))
2	1	reservoir	Tris	0.1 (M)
3	1	drop	protein	4-10 (mg/ml)
4	1	drop	HEPES	30 (mM)
5	1	drop		80 (mM)