2LI5

NMR structure of Atg8-Atg7C30 complex

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Varian	INOVA	Varian INOVA	800
2	Varian	INOVA	Varian INOVA	600
3	Varian	INOVA	Varian INOVA	600
4	Varian	INOVA	Varian INOVA	500

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	1	3D 1H-13C NOESY aromatic
11	1	1	3D CBCA(CO)NH
12	1	2	2D 1H-15N HSQC
13	1	2	3D HNCO
14	1	2	3D HNCACB
15	1	2	3D HNCACB
16	1	2	3D 1H-15N NOESY
17	1	2	3D C(CO)NH
18	1	2	2D 1H-13C HSQC aliphatic
19	1	2	3D 1H-13C NOESY aliphatic
2	1	1	2D 1H-13C HSQC aliphatic
20	1	2	2D 1H-13C HSQC aromatic
21	1	2	3D HCCH-TOCSY aliphatic
22	1	1	3D HCCH-TOCSY aromatic
23	1	2	3D 1H-13C NOESY aromatic
3	1	1	3D 1H-15N NOESY
4	1	1	3D 1H-13C NOESY aliphatic
5	1	1	3D HCCH-TOCSY aliphatic
6	1	1	2D HbCbCgCdHd
7	1	1	2D 1H-13C HSQC aromatic
8	1	1	3D HNCA
9	1	1	3D HNCO

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.8	ambient	298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	19