2LDB
STRUCTURE DETERMINATION AND REFINEMENT OF BACILLUS STEAROTHERMOPHILUS LACTATE DEHYDROGENASE
Experimental procedure
| Spacegroup name | P 61 |
| Unit cell lengths | 86.800, 86.800, 356.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 3.000 |
| R-factor | 0.26 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.043 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 * |
| High resolution limit [Å] | 3.000 * |
| Rmerge | 0.109 * |
| Total number of observations | 76407 * |
| Number of reflections | 24793 * |
| Completeness [%] | 79.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 36 (%sat) | |
| 2 | 1 | 1 | PIPES | 50 (mM) | |
| 3 | 1 | 1 | NADH | 20 (mM) | |
| 4 | 1 | 1 | FDP | 20 (mM) |
