2L6U
Solution NMR Structure of Med25(391-543) Comprising the Activator-Interacting Domain (ACID) of Human Mediator Subuniti 25. Northeast Structural Genomics Consortium Target HR6188A
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | 900 | |
| 2 | Varian | INOVA | 750 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 1H-15N HSQC |
| 10 | 1 | 1 | 2D 1H-13C CT-HSQC aliphatic |
| 11 | 1 | 1 | 2D 1H-13C CT-HSQC aromatic |
| 12 | 1 | 1 | 1D 15N T1 |
| 13 | 1 | 1 | 1D 15N T2 |
| 14 | 1 | 1 | 3D (H)CCH-TOCSY aliphatic |
| 15 | 1 | 1 | 3D (H)CCH-COSY aliphatic |
| 16 | 1 | 1 | 3D (H)CCH-COSY aromatic |
| 17 | 1 | 1 | 2D 1H-15N LR-HSQC for histidines |
| 18 | 1 | 1 | 2D (HB)CB(CGCDCE)HDHE |
| 19 | 1 | 2 | 2D 1H-13C CT-HSQC methyl |
| 2 | 1 | 1 | 2D 1H-13C HSQC aliphatic |
| 3 | 1 | 1 | 3D HNCO |
| 4 | 1 | 1 | 3D CBCA(CO)NH |
| 5 | 1 | 1 | 3D HNCACB |
| 6 | 1 | 1 | 3D HN(CA)CO |
| 7 | 1 | 1 | 3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY |
| 8 | 1 | 1 | 3D HBHA(CO)NH |
| 9 | 1 | 1 | 3D HNCA |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.5 | AMBIENT | 298 |
Conformers
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
