2L5Z

NMR structure of the A730 loop of the Neurospora VS ribozyme

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Varian	INOVA	Varian INOVA	500
2	Varian	INOVA	Varian INOVA	600
3	Varian	INOVA	Varian INOVA	800

Experiment

experiment id	conditions id	solution id	Experiment type
1	2	6	2D 1H-13C CT-HSQC
10	1	5	2D H(NCCC)H for uracil and cytosine residues
11	1	5	2D H(NC)-TOCSY-(C)H for guanosine residues
12	1	5	2D (H)N(C)-TOCSY-(C)H for adenosine residues
13	1	3	2D 1H-15N CPMG-NOESY
14	2	4	2D 1H 15N HMQC optimized for transfers via J=7.0 Hz and J=21 Hz
15	1	3	2D HNN-COSY
16	2	2	2D DQF-COSY
17	2	6	3D HCCH-E.COSY
18	1	7	2D imino-optimized 2D 1H-15N HSQC
19	2	8	Spin-state selective experiments
2	2	6	2D 1H-13C HMQC
20	2	6	Spin-state selective experiments
3	2	6	2D 1H-15N MQ-(HC)N(C)H
4	2	6	3D CT-HCCH-COSY
5	2	6	3D HCCH-TOCSY
6	2	6	3D 13C-edited HMQC-NOESY
7	1	1	1D flip-back watergate 1H
8	1	1	2D flip-back watergate NOESY
9	1	3	2D imino- and amino-optimized 2D 1H-15N HSQC

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.5	ambient	288
2	6.5	ambient	288

Conformers

Conformers Calculated Total Number	500
Conformers Submitted Total Number	21